lc3 expression Search Results


plasmids expressing rfp-lc3  (Shanghai GenePharma)
90
Shanghai GenePharma plasmids expressing rfp-lc3
Thioredoxin‐interacting protein <t>(TXNIP)</t> increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm
Plasmids Expressing Rfp Lc3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+expression/plasmids+expressing+rfp+lc3/pmc06492710-80-4-13
Average 90 stars, based on 1 article reviews
plasmids expressing rfp-lc3 - by Bioz Stars, 2026-10
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pbabe-egfp-mcherry-lc3  (BioVector Inc)
90
BioVector Inc pbabe-egfp-mcherry-lc3
Thioredoxin‐interacting protein <t>(TXNIP)</t> increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm
Pbabe Egfp Mcherry Lc3, supplied by BioVector Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+expression/gfp+lc3+expression+plasmid/pmc04358430-50-12-16
Average 90 stars, based on 1 article reviews
pbabe-egfp-mcherry-lc3 - by Bioz Stars, 2026-10
90/100 stars
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lc3 expression  (MBL International)
90
MBL International lc3 expression
Thioredoxin‐interacting protein <t>(TXNIP)</t> increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm
Lc3 Expression, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+expression/lc3+expression/pm20555322-63-9-10
Average 90 stars, based on 1 article reviews
lc3 expression - by Bioz Stars, 2026-10
90/100 stars
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atg5 autophagy-related marker  (SCAPS GmbH)
90
SCAPS GmbH atg5 autophagy-related marker
Thioredoxin‐interacting protein <t>(TXNIP)</t> increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm
Atg5 Autophagy Related Marker, supplied by SCAPS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+expression/protein+expression+of+autophagy+related+proteins+lc3++atg5++and+beclin1/pm30767218-182-19-13
Average 90 stars, based on 1 article reviews
atg5 autophagy-related marker - by Bioz Stars, 2026-10
90/100 stars
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gfp lc3 expression plasmid  (Aladin Enterprises Inc)
86
Aladin Enterprises Inc gfp lc3 expression plasmid
Thioredoxin‐interacting protein <t>(TXNIP)</t> increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm
Gfp Lc3 Expression Plasmid, supplied by Aladin Enterprises Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+expression/expression+gfp+lc3+plasmid/pm20830296-151-8-11
Average 86 stars, based on 1 article reviews
gfp lc3 expression plasmid - by Bioz Stars, 2026-10
86/100 stars
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lc3 expression  (Kuang Lung Shing)
86
Kuang Lung Shing lc3 expression
Thioredoxin‐interacting protein <t>(TXNIP)</t> increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm
Lc3 Expression, supplied by Kuang Lung Shing, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+expression/expression+lc3/10__1177_slash_09731296241283126-33-16-38
Average 86 stars, based on 1 article reviews
lc3 expression - by Bioz Stars, 2026-10
86/100 stars
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Image Search Results


Thioredoxin‐interacting protein (TXNIP) increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm

Journal: CNS Neuroscience & Therapeutics

Article Title: Thioredoxin‐interacting protein induced α‐synuclein accumulation via inhibition of autophagic flux: Implications for Parkinson's disease

doi: 10.1111/cns.12721

Figure Lengend Snippet: Thioredoxin‐interacting protein (TXNIP) increased in the midbrain of A53T mice and in Parkinson's disease (PD) cellular model. (A) Western blot analysis of TXNIP in 5‐month‐old WT and A53T mice. (B) Western blot analysis of TXNIP in HEK293 cells transfected with α‐synuclein plasmid. (C) The supernatant of HEK293 cells transfected with vector or α‐synuclein plasmid was collected to stimulate SH‐SY5Y cells for 48 hours. Then, TXNIP was detected by Western blot. HEK293 cells were cultured in 6‐well or 96‐well plates and transfected with TXNIP or α‐synuclein plasmid for 48 hours. (D) Western blot analysis of TXNIP. (E) Cytotoxicity was measured by MTT conversion. (F) Cell apoptosis was detected by TUNEL staining. *P<.05, **P<.01 vs WT mice or vector‐transfected cells. n=4 in (A); n=3 in (B‐F). Scale bar, 50 μm

Article Snippet: Plasmids and transfection The plasmids expressing TXNIP, RFP‐LC3, and ATP13A2 were purchased from GenePharma (Suzhou, China).

Techniques: Western Blot, Transfection, Plasmid Preparation, Cell Culture, TUNEL Assay, Staining

Thioredoxin‐interacting protein (TXNIP) blocked autophagic flux. (A) HEK293 cells were cotransfected with RFP‐LC3/TXNIP plasmids. LC3 spots were detected by fluorescence microscopy. To inhibit lysosome, HEK293 cells were treated with 20 μmol L−1 NH 4Cl from 7 to 48 hours after TXNIP transfection. (B‐D) LC3, p62, p‐AMPK, AMPK, and Beclin 1 were determined by Western blot analysis. *P<0.05, **P<0.01 vs vector‐transfected cells. n=3. Scale bar, 10 μm

Journal: CNS Neuroscience & Therapeutics

Article Title: Thioredoxin‐interacting protein induced α‐synuclein accumulation via inhibition of autophagic flux: Implications for Parkinson's disease

doi: 10.1111/cns.12721

Figure Lengend Snippet: Thioredoxin‐interacting protein (TXNIP) blocked autophagic flux. (A) HEK293 cells were cotransfected with RFP‐LC3/TXNIP plasmids. LC3 spots were detected by fluorescence microscopy. To inhibit lysosome, HEK293 cells were treated with 20 μmol L−1 NH 4Cl from 7 to 48 hours after TXNIP transfection. (B‐D) LC3, p62, p‐AMPK, AMPK, and Beclin 1 were determined by Western blot analysis. *P<0.05, **P<0.01 vs vector‐transfected cells. n=3. Scale bar, 10 μm

Article Snippet: Plasmids and transfection The plasmids expressing TXNIP, RFP‐LC3, and ATP13A2 were purchased from GenePharma (Suzhou, China).

Techniques: Fluorescence, Microscopy, Transfection, Western Blot, Plasmid Preparation

ATP13A2 attenuated α‐synuclein accumulation induced by Thioredoxin‐interacting protein (TXNIP). (A) HEK293 cells were transfected with α‐synuclein plasmid. α‐Synuclein was detected by Western blot analysis. (B) TXNIP and ATP13A2 were cotransfected into α‐syn‐transfected HEK293 cells to explore their effects on α‐synuclein accumulation. α‐Synuclein was detected by Western blot analysis. *P<0.05 vs vector‐transfected cells; # P<0.05 vs TXNIP‐transfected cells. n=3

Journal: CNS Neuroscience & Therapeutics

Article Title: Thioredoxin‐interacting protein induced α‐synuclein accumulation via inhibition of autophagic flux: Implications for Parkinson's disease

doi: 10.1111/cns.12721

Figure Lengend Snippet: ATP13A2 attenuated α‐synuclein accumulation induced by Thioredoxin‐interacting protein (TXNIP). (A) HEK293 cells were transfected with α‐synuclein plasmid. α‐Synuclein was detected by Western blot analysis. (B) TXNIP and ATP13A2 were cotransfected into α‐syn‐transfected HEK293 cells to explore their effects on α‐synuclein accumulation. α‐Synuclein was detected by Western blot analysis. *P<0.05 vs vector‐transfected cells; # P<0.05 vs TXNIP‐transfected cells. n=3

Article Snippet: Plasmids and transfection The plasmids expressing TXNIP, RFP‐LC3, and ATP13A2 were purchased from GenePharma (Suzhou, China).

Techniques: Transfection, Plasmid Preparation, Western Blot

ATP13A2 improved dysfunction of autophagy induced by Thioredoxin‐interacting protein (TXNIP). (A‐C) ATP13A2, LC3, and p62 were detected by Western blot analysis. *P<0.05, **P<0.01 vs vector‐transfected cells; # P<0.05 vs TXNIP‐transfected cells. n=3

Journal: CNS Neuroscience & Therapeutics

Article Title: Thioredoxin‐interacting protein induced α‐synuclein accumulation via inhibition of autophagic flux: Implications for Parkinson's disease

doi: 10.1111/cns.12721

Figure Lengend Snippet: ATP13A2 improved dysfunction of autophagy induced by Thioredoxin‐interacting protein (TXNIP). (A‐C) ATP13A2, LC3, and p62 were detected by Western blot analysis. *P<0.05, **P<0.01 vs vector‐transfected cells; # P<0.05 vs TXNIP‐transfected cells. n=3

Article Snippet: Plasmids and transfection The plasmids expressing TXNIP, RFP‐LC3, and ATP13A2 were purchased from GenePharma (Suzhou, China).

Techniques: Western Blot, Plasmid Preparation, Transfection

Thioredoxin‐interacting protein (TXNIP) resulted in DA neuron loss in mouse midbrain and induced autophagic dysfunction. (A) TH immunohistochemistry and stereological counts of TH‐positive cells in the midbrain. (B‐D) TXNIP, LC3, p62, ATP13A2, and α‐synuclein were detected by Western blot analysis. *P<.05 vs control mice. n=4. Scale bar, 200 μm

Journal: CNS Neuroscience & Therapeutics

Article Title: Thioredoxin‐interacting protein induced α‐synuclein accumulation via inhibition of autophagic flux: Implications for Parkinson's disease

doi: 10.1111/cns.12721

Figure Lengend Snippet: Thioredoxin‐interacting protein (TXNIP) resulted in DA neuron loss in mouse midbrain and induced autophagic dysfunction. (A) TH immunohistochemistry and stereological counts of TH‐positive cells in the midbrain. (B‐D) TXNIP, LC3, p62, ATP13A2, and α‐synuclein were detected by Western blot analysis. *P<.05 vs control mice. n=4. Scale bar, 200 μm

Article Snippet: Plasmids and transfection The plasmids expressing TXNIP, RFP‐LC3, and ATP13A2 were purchased from GenePharma (Suzhou, China).

Techniques: Immunohistochemistry, Western Blot, Control